Animal RanBP1 nuclear export and cargo dissociation mechanisms are surprisingly different from yeast, due to mutations of critical residues, leading to greater nuclear transport efficiency and higher energy cost.
New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.
The social amoeba Dictyostelium discoideum employs a terpene synthase-cytochrome P450 metabolic gene cluster to produce a novel trisnorsesquiterpene discodiene.
During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.
The nutrients available in some tumors and the factors that influence tumor nutrient availability are characterized, which provides insight into the metabolic constraints of the tumor microenvironment.
Both exogenous and endogenous genes can be highly activated in cells by a new dCas9-based activator, CRISPR-assisted trans enhancer, that can be used to activate genes for biomedical applications.
Ruben M Atilho, Gayan Mirihana Arachchilage ... Ronald R Breaker
Many bacteria use a tiny riboswitch aptamer to sense the thiamin precursor HMP-PP to regulate the production of another thiamin precursor HET-P to efficiently biosynthesize this essential coenzyme.
Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.
