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By performing ¹⁵N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.

The 26S proteasome lid subcomplex acts as an external scaffold whose interactions with the ATPase motor stabilize the substrate-engagement-competent state, and control conformational changes upon engagement for subsequent degradation steps.

Through visualization of directly-labeled RhoGTPase both in vitro and in vivo, RhoGDI is found to spatiotemporally regulate RhoGTPase activity through the extraction of active RhoGTPase.

New methods to directly visualize Rho GTPases reveal how a protein called RhoGDI regulates the activity of these 'molecular switches' at the plasma membrane.

GRAMD1 proteins sense a transient expansion of the accessible pool of plasma membrane cholesterol and facilitate its transport to the endoplasmic reticulum at ER-PM contact sites.

Cyclin-dependent kinase 5 regulates the circadian clock involving phosphorylation of the PER2 protein.

Outer membrane β-barrel proteins in Gram-negative bacteria are assembled within the lumen of the BamA β-barrel.

Post-translational modifications regulate transient protein-protein interactions to disrupt Ubiquitin enzyme/ligase complexes, a strategy used by Shigella to inactivate the host immune response.

Relying on RNA fragments derived from degradation or partial template copying, non-enzymatic primer extension with strand displacement offers a novel and prebiotically plausible approach to RNA self-replication.

Profilin release from the filament end controls the speed of actin growth at physiological conditions.