Senem Ntourmas, Martin Sachs ... Dominic B Bernkopf
Biochemical detection of endogenous, paralog-specific DVL2 complexes, molecular characterization of underlying protein domains and functional evidence for their importance extend the knowledge of Wnt pathway regulation through DVL2 biomolecular condensates.
Stress-regulated secretion of an intestinal peptide positively regulates the antioxidant response by promoting neuropeptide release from the nervous system, defining a gut-to-brain-to-gut endocrine axis in the oxidative stress response.
Aging reduces the potassium M current in sympathetic motor neurons, resulting in a hyperactive phenotype that can be reversed by pharmacological activation of these channels.
Anne Drougard, Eric H Ma ... John Andrew Pospisilik
High-fat ingestion triggers microglial metabolic activation within a single feeding cycle, and enhances whole-body metabolism and memory by converting fatty acids into protective substrates for surrounding neurons.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
Gradual downregulation of each of the subunits of the evolutionary-conserved exocyst holocomplex shows that the complex is required for biogenesis, maturation, and exocytosis of secretory granules in Drosophila salivary glands.
The automated workflow significantly enhances the precision and scalability of cell cycle analysis, enabling high-throughput studies in both non-adherent and adherent cells for advanced cancer research and drug development.
Fatima Tleiss, Martina Montanari ... C Leopold Kurz
Bacterial location quantifications highlights how Drosophila melanogaster larvae discriminate bacteria to isolate and later eliminate pathogens in the anterior midgut through coordinated mechanisms involving reactive oxygen species (ROS) and antimicrobial peptides (AMPs).
