A fully computationally designed SaCas9 variant expands PAM recognition to NNNRRT, achieving up to 116-fold higher editing at noncanonical sites while matching the performance of experimentally evolved variants.
The first all-atom models of the mycobacterial outer membrane reveal how lipid organization and asymmetry generate a structurally heterogeneous barrier that underlies its unique permeability properties.
Real-time nanopore transcriptomics enables early detection of expression changes and sample quality, offering a rapid, cost-effective strategy for experimental quality control and transcriptomic analysis.
Mammalian cells dynamically scale translation initiation to match distinct elongation rates, preventing ribosome crowding and preserving protein synthesis homeostasis across diverse transcripts.
