Easy-to-use image analysis software enables single cell quantitation of cell types and division rates in complex 3D tissues including living Drosophila brains, mouse embryos and Zebrafish organoids.
Michael Berger, Naubahar Shahryar Agha, Alexander Gail
The novel Reach Cage allows neurophysiology studies of structured behavior with unrestrained Rhesus macaques showing that the frontoparietal reach network is selective for reach goals outside the immediately reachable space.
Eli M Carrami, Sahand Sharifzadeh ... Ahmed A Ahmed
A robust library preparation and variant calling strategy (DigiPico/MutLX) eliminates false positive single nucleotide variants from picogram quantities of DNA following whole genome amplification.
Haojiang Luan, Alexander Kuzin ... Benjamin H White
A genetic method allows neurons to be individually identified and characterized by combining information about both their developmental origins and their mature patterns of gene expression.
Edgar M Medina, Kristyn A Robinson ... Nicolas E Buchler
Spizellomycespunctatusis a genetically tractable chytrid and model organism for comparative cell biology for understanding evolution of the cell cycle, actin dynamics, and cellularization in fungi and early eukaryotes.
David S White, Marcel P Goldschen-Ohm ... Baron Chanda
A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.
Chih-Chieh (Jay) Yu, Nicholas C Barry ... Edward S Boyden
Fixed, intact animals of C. elegans can be physically expanded with high isotropy, to enable super-resolved imaging of general proteins and nucleic acids throughout the organism, on conventional microscopes.
A new user-intervention-free classification, using single-fluorescent markers, measures conventionally unmeasureable phenotypes in early stages during clathrin-mediated endocytosis.
The forces that multicellular tumor aggregates exert on their environment lead to non-linear, scale-invariant tissue deformations far away from the tumor, which can be exploited to quantify its collective contractility.
