Single-cell RNA-sequencing resolves the transcriptional landscape of asexual development in Toxoplasma gondii, revealing concerted genetic programs to Plasmodiumfalciparum and a novel transcriptional factor that controls antigen switching.
Generation of stable transgenic opsin lines together with in vivo calibration of their efficacy using behavioural and electrophysiological assays constitutes a novel optogenetic toolkit in zebrafish.
Arjun Khakhar, Colby G Starker ... Daniel F Voytas
A fungal bioluminescence pathway can be reconstituted in planta to create luminescence in many plant species without external substrate addition, and be used to design customizable reporters of gene-expression.
Jérôme Dockès, Russell A Poldrack ... Gaël Varoquaux
Full-text analysis of the human brain-imaging literature can predict the spatial distribution of observations in the brain given the description of a subject of study.
Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.
Chu Hsien Lim, Prameet Kaur ... Nicholas S Tolwinski
An optogenetic approach has been developed to model Alzheimer's disease allowing light-induced Amyloid-β aggregation and tested in three model organisms, Drosophila, C. elegans and D. rerio.
David J Forsthoefel, Nicholas I Cejda ... Phillip A Newmark
Application of laser-capture microdissection to planarian intestinal tissue provides a new tool for analysis of tissue-specific gene expression in flatworms, and a new resource to advance investigations of gastrointestinal regeneration.
Sharing synthetic datasets that mimic original datasets that could not otherwise be made publicly available can help ensure reproducibility and facilitate data exploration while maintaining participant privacy.
Robinson Arce-Molina, Francisca Cortés-Molina ... Alejandro San Martín
A user-friendly genetically-encoded fluorescent sensor of improved dynamic range is introduced to facilitate the study of mitochondrial metabolism and diversity.
