Browse our Tools and Resources

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    1. Neuroscience

    MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

    Thomas Templier
    The augmentation of resin-embedded sample blocks with magnetic resin allows remote actuation of ultrathin sections and deposition at high packing density onto silicon wafers for correlative light and electron microscopy.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries

    Michael Luke Carlson, R Greg Stacey ... Franck Duong Van Hoa
    Trapping of the cell envelope proteome into water-soluble peptidisc library allows high-throughput definition of the membrane protein interactome.
    1. Cell Biology
    2. Physics of Living Systems

    KymoButler, a deep learning software for automated kymograph analysis

    Maximilian AH Jakobs, Andrea Dimitracopoulos, Kristian Franze
    KymoButler is a machine learning approach towards the reliable, quick, and fully automated analysis of the dynamics of fluorescently labelled particles in living cells.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    The origins and relatedness structure of mixed infections vary with local prevalence of P. falciparum malaria

    Sha Joe Zhu, Jason A Hendry ... Gil McVean
    Variation in the rate of mixed infections by malaria parasites and the relatedness structure among infecting strains reveals diversity in local epidemiological processes.
    1. Developmental Biology

    In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer

    Li He, Richard Binari ... Norbert Perrimon
    A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology

    Amal Alex, Valentina Piano ... Stefano Maffini
    An electroporation-based method allows the direct delivery of recombinant proteins into mammalian cells for in vivo functional studies.
    1. Cell Biology

    In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation

    Alison K Gillingham, Jessie Bertram ... Sean Munro
    Relocation of Rab, Ras and Rho family GTPases to the surface of mitochondria enables efficient identification of their effectors, exchange factors and GAPs by proximity biotinylation.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Identifying gene expression programs of cell-type identity and cellular activity with single-cell RNA-Seq

    Dylan Kotliar, Adrian Veres ... Pardis C Sabeti
    A novel algorithm, consensus non-negative matrix factorization (cNMF), accurately identifies gene expression programs underlying cell-type identity and cellular activities from single-cell RNA-Seq data.
    1. Ecology

    Genetic transformation of the dinoflagellate chloroplast

    Isabel C Nimmo, Adrian C Barbrook ... Christopher J Howe
    Successful stable transformation of the dinoflagellate chloroplast genome.
    1. Physics of Living Systems

    A microfluidic device for inferring metabolic landscapes in yeast monolayer colonies

    Zoran S Marinkovic, Clément Vulin ... Pascal Hersen
    Metabolic gradients underlie gene expression patterns within spatially controlled expanding 2D microbial colonies.