Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
Adi Naamati, James C Williamson ... Nicholas J Matheson
Identification and characterisation of proteins and processes regulated by HIV in primary human CD4+ T cells using an HIV reporter virus for one-step, antibody-free magnetic selection.
As the first fully genetically encoded method, PARIS allows cell-specific, long-term, repeated measurements of gap junctional coupling with high spatiotemporal resolution, facilitating its study in both health and disease.
Visualization methods display the dynamics of the currents in conductance-based model neurons, and show how their contribution changes in response to perturbation.
Leave-One-Trial-Out (LOTO) is a general, efficient and easily implementable approach for inferring trial-by-trial measures of computational model parameters in order to link these measures to neural mechanisms.
Kathryn M Tabor, Gregory D Marquart ... Harold A Burgess
A new resource of transgenic zebrafish lines facilitates precise targeting of neuronal cell-types within the brain, supported by online software for visualizing transgene expression patterns.
Phototrophic growth laws are elucidated by combining computational modeling and experiments for quantitative evaluation of cellular physiology, morphology and proteome allocation across a wide range of light conditions.
A new computational framework provides a flexible and general approach for single and collective biological motion characterisation and phenotyping ideally suited for high-throughput timelapse screens.
Joshua T Vogelstein, Eric W Bridgeford ... Cencheng Shen
Multiscale Graph Correlation, an interpretable hypothesis test with strong theoretical guarantees for discerning relationships in complex data, requires about half the sample size as other methods, whilst maintaining computational tractability.
