BK and CaV1.3 channels interact early during biogenesis, assembling intracellularly before membrane localization, indicating coordinated processes for functional coupling.
Modestas Matusevicius, Robin A Corey ... Simone Weyand
Cryo-EM structures of human aquaporin AQP2 bound to the anti-trypanosomal drugs pentamidine and melarsoprol identify the molecular mechanism for drug-resistant sleeping sickness.
Single-molecule tracking of transcription factors in living cells revealed how mutations that make short activation domains stronger increased the fraction of transcription factor molecules bound to chromatin and led to longer residence times on chromatin.
The stability of ribonucleoprotein complexes of SARS-CoV-2 nucleocapsid proteins differs among variants of concern and the viral evolution of nucleocapsid protein can be understood in the framework of fuzzy complexes.
Marta Wojnowska, Takeaki Wajima ... Ulrich Schwarz-Linek
The T-shaped fold of an abundant surface protein ties streptococci to collagens, an interaction that enhances biofilm such as often found in invasive infections.
Teresa Almeida, Eleanor Hargreaves ... Igor Barsukov
Structural analysis reveals a two-step EB1-SxIP binding mechanism and identifies ligand mutations that greatly enhance affinity, offering potential therapeutic avenues for targeting microtubule plus end interactions.
Samantha J Cassell, Weiyan Li ... Jonathan R Whicher
Cryo-EM and electrophysiology reveal the mechanism of SK2 channel modulation by the bee toxin apamin and small molecules, providing insights that enable the rational design of targeted SK2 channel therapeutics.
Hydration modulates aromatic interactions, explaining why Tyr is a stronger sticker than Phe in aqueous environments like protein condensates but not in the cores of folded proteins.
The path of potassium through an ATP-dependent membrane pump is elucidated with a high-resolution structure within a lipid bilayer together with mutational analysis at key points along this pathway.
